Target DNA bending by the Mu transpososome promotes careful transposition and prevents its reversal

Abstract

The transposition of bacteriophage Mu serves as a model system for understanding DDE transposases and integrases. All available structures of these enzymes at the end of the transposition reaction, including Mu, exhibit significant bends in the transposition target site DNA. Here we use Mu to investigate the ramifications of target DNA bending on the… (More)
DOI: 10.7554/eLife.21777

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