Taraxalisin – a serine proteinase from dandelion Taraxacum officinale Webb s.l

@article{Rudenskaya1998TaraxalisinA,
  title={Taraxalisin – a serine proteinase from dandelion Taraxacum officinale Webb s.l},
  author={G. N. Rudenskaya and A. M. Bogacheva and A. Preusser and A. V. Kuznetsova and Ya.E. Dunaevsky and B. N. Golovkin and V. M. Stepanov},
  journal={FEBS Letters},
  year={1998},
  volume={437}
}
  • G.N. Rudenskaya, A. M. Bogacheva, +4 authors V.M. Stepanov
  • Published 1998
  • Medicine, Chemistry
  • FEBS Letters
  • Latex of dandelion roots contains a serine proteinase that hydrolyzes a chromogenic peptide substrate Glp‐Ala‐Ala‐Leu‐pNA optimally at pH 8.0. Maximal activity of the proteinase in the roots is attained in April, at the beginning of plant development after the winter period. The protease was isolated by ammonium sulfate precipitation of the root extract followed by affinity chromatography on a Sepharose‐Ala‐Ala‐Leu‐mrp and gel filtration on Superose 6R performed in FPLC regime. Pure serine… CONTINUE READING
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