TaqManR proximity ligation technology for the detection of heterodimeric adhesion receptors on lymphocytes.

@article{Gehwolf2014TaqManRPL,
  title={TaqManR proximity ligation technology for the detection of heterodimeric adhesion receptors on lymphocytes.},
  author={R. Gehwolf and E. Band and A. Trost and B. Iglseder and E. Trinka and E. Haschke-Becher and J. Kraus and A. Harrer},
  journal={Journal of immunological methods},
  year={2014},
  volume={404},
  pages={
          81-6
        }
}
  • R. Gehwolf, E. Band, +5 authors A. Harrer
  • Published 2014
  • Medicine, Biology
  • Journal of immunological methods
  • TaqMan(R) proximity ligation technology (TaqMan(R) PLA) is an innovative advancement of immuno PCR. It allows a fast and quantitative detection of vicinal proteins or protein-protein interactions from cell lysates by combining antibody-antigen binding with a real-time PCR detection. We tested if TaqMan(R) PLA also was applicable to investigate and relatively quantitate adhesion receptor heterodimers such as the alpha-4/beta-1 integrin on the surface of intact cells. Both, alpha-4, beta-1 and… CONTINUE READING
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