Tandem Spinach Array for mRNA Imaging in Living Bacterial Cells.

@article{Zhang2015TandemSA,
  title={Tandem Spinach Array for mRNA Imaging in Living Bacterial Cells.},
  author={Jichuan Zhang and Jingyi Fei and Benjamin J. Leslie and Kyu Young Han and Thomas E Kuhlman and Taekjip Ha},
  journal={Scientific reports},
  year={2015},
  volume={5},
  pages={17295}
}
Live cell RNA imaging using genetically encoded fluorescent labels is an important tool for monitoring RNA activities. A recently reported RNA aptamer-fluorogen system, the Spinach, in which an RNA aptamer binds and induces the fluorescence of a GFP-like 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) ligand, can be readily tagged to the RNA of interest. Although the aptamer-fluorogen system is sufficient for imaging highly abundant non-coding RNAs (tRNAs, rRNAs, etc.), it performs… CONTINUE READING
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