Taking stem cells to the clinic: Major challenges

@article{Bongso2008TakingSC,
  title={Taking stem cells to the clinic: Major challenges},
  author={Ariff Bongso and Chui-Yee Fong and Kalamegam Gauthaman},
  journal={Journal of Cellular Biochemistry},
  year={2008},
  volume={105}
}
Stem cell therapy offers tremendous promise in the treatment of many incurable diseases. A variety of stem cell types are being studied but human embryonic stem cells (hESCs) appear to be the most versatile as they are pluripotent and can theoretically differentiate into all the tissues of the human body via the three primordial germ layers and the male and female germ lines. Currently, hESCs have been successfully converted in vitro into functional insulin secreting islets, cardiomyocytes, and… Expand
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References

SHOWING 1-10 OF 56 REFERENCES
Isolation of amniotic stem cell lines with potential for therapy
TLDR
The isolation of human and rodent amniotic fluid–derived stem (AFS) cells that express embryonic and adult stem cell markers are reported and examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels. Expand
Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice.
TLDR
This study is the first report to demonstrate that human ES cells are prone to generate primitive, undifferentiated tumors in human fetal tissue grafts in SCID mice and raises a potential safety concern for using human ES cell-derived cell products in humans. Expand
Highly sensitive biosafety model for stem-cell-derived grafts.
TLDR
This model will facilitate the generation of homogenous non-tumorigenic cell populations, and will help to integrate standardized safety systems into the application of stem-cell-derived grafts for clinical purposes. Expand
History and perspective of stem cell research.
  • A. Bongso, M. Richards
  • Biology, Medicine
  • Best practice & research. Clinical obstetrics & gynaecology
  • 2004
TLDR
Stem cell research opens-up the new field of 'cell-based therapies' and, as such, several safety measures have also to be evaluated. Expand
Regulatory Issues for Personalized Pluripotent Cells
TLDR
The outstanding long‐term potential of iPSCs and their relative freedom from the ethical concerns raised by both ESCs and NT‐ESCs makes direct reprogramming an exceptionally promising approach to advancing research and providing therapies in the field of regenerative medicine. Expand
Differentiation of Encapsulated Embryonic Stem Cells After Transplantation
TLDR
Embryonic stem cells do differentiate, although along multiple pathways, both when transplanted and maintained in culture, just as nonencapsulated ESC do when removed from their feeder layer. Expand
Teratoma formation leads to failure of treatment for type I diabetes using embryonic stem cell-derived insulin-producing cells.
TLDR
It is demonstrated that reversal of hyperglycemia by transplantation of ES cell-derived insulin-producing cells is possible and the risk of teratoma formation would need to be eliminated beforeES cell-based therapies for the treatment of diabetes are considered. Expand
Producing primate embryonic stem cells by somatic cell nuclear transfer
TLDR
A modified SCNT approach is used to produce rhesus macaque blastocysts from adult skin fibroblasts, and two ES cell lines are isolated from these embryos, representing successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrating proof-of-concept for therapeutic cloning in primates. Expand
Human Adult Marrow Cells Support Prolonged Expansion of Human Embryonic Stem Cells in Culture
TLDR
The well‐studied hMSCs (and this animal cell‐ and serum‐free system) may provide a clinically and ethically feasible method to expand hES cells for novel cell therapies and this system may help to identify cytokines and adhesion molecules that are required for the self‐renewal of h ES cells. Expand
Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views
TLDR
Critically evaluate the literature describing the plasticity of MSCs and offer insight into how the molecular and functional heterogeneity of this cell population, which reflects the complexity of marrow stroma as an organ system, may confound interpretation of their transdifferentiation potential. Expand
...
1
2
3
4
5
...