Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing.

@article{Mazin2000TailedDD,
  title={Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing.},
  author={A. V. Mazin and Elena Zaitseva and Patrick Sung and Stephen C Kowalczykowski},
  journal={The EMBO journal},
  year={2000},
  volume={19 5},
  pages={1148-56}
}
The repair of potentially lethal DNA double-stranded breaks (DSBs) by homologous recombination requires processing of the broken DNA into a resected DNA duplex with a protruding 3'-single-stranded DNA (ssDNA) tail. Accordingly, the canonical models for DSB repair require invasion of an intact homologous DNA template by the 3'-end of the ssDNA, a characteristic that the bacterial pairing protein RecA possesses. Unexpectedly, we find that for the eukaryotic homolog, Rad51 protein, the 5'-end of… CONTINUE READING