Tail morphogenesis in the ascidian, Ciona intestinalis, requires cooperation between notochord and muscle.

@article{Gregorio2002TailMI,
  title={Tail morphogenesis in the ascidian, Ciona intestinalis, requires cooperation between notochord and muscle.},
  author={Anna Di Gregorio and Richard M Harland and Michael Levine and Elena M Silva Casey},
  journal={Developmental biology},
  year={2002},
  volume={244 2},
  pages={385-95}
}
We present evidence that notochord and muscle differentiation are crucial for morphogenesis of the ascidian tail. We developed a novel approach for embryological manipulation of the developing larval tissues using a simple method to introduce DNA into Ciona intestinalis and the several available tissue-specific promoters. With such promoters, we misexpressed the Xenopus homeobox gene bix in notochord or muscle of Ciona embryos as a means of interfering with development of these tissues. Ciona… CONTINUE READING
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