Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C

@article{Cheng2006TaggingRP,
  title={Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C},
  author={Qing Cheng and Sharon Stone-Elander and Elias S. J. Arn{\'e}r},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={604-613}
}
Selenocysteine (Sec; U in one-letter code) is the twenty-first naturally occurring amino acid, with a selenium atom that gives this cysteine (Cys) homolog unique biochemical properties, including a high nucleophilicity and significant reactivity with electrophilic agents. This can be used in biotechnological Sec-dependent applications. Here, we describe how… CONTINUE READING
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