Tagetitoxin inhibits RNA polymerase through trapping of the trigger loop.

@article{Artsimovitch2011TagetitoxinIR,
  title={Tagetitoxin inhibits RNA polymerase through trapping of the trigger loop.},
  author={Irina Artsimovitch and Vladimir Svetlov and Sondra Maureen Nemetski and Vitaly Epshtein and Timothy Cardozo},
  journal={The Journal of biological chemistry},
  year={2011},
  volume={286 46},
  pages={40395-400}
}
Tagetitoxin (Tgt) inhibits multisubunit chloroplast, bacterial, and some eukaryotic RNA polymerases (RNAPs). A crystallographic structure of Tgt bound to bacterial RNAP apoenzyme shows that Tgt binds near the active site but does not explain why Tgt acts only at certain sites. To understand the Tgt mechanism, we constructed a structural model of Tgt bound to the transcription elongation complex. In this model, Tgt interacts with the β' subunit trigger loop (TL), stabilizing it in an inactive… CONTINUE READING