Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components.

@article{Gan2002TagmediatedIO,
  title={Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components.},
  author={Xiang Gan and Madoka Kitakawa and Ken-ichi Yoshino and Noriko Oshiro and Kazuyoshi Yonezawa and Katsumi Isono},
  journal={European journal of biochemistry},
  year={2002},
  volume={269 21},
  pages={5203-14}
}
Mitochondrial ribosomal proteins (mrps) of the budding yeast, Saccharomyces cerevisiae, have been extensively characterized genetically and biochemically. However, the list of the genes encoding individual mrps is still not complete and quite a few of the mrps are only predicted from their similarity to bacterial ribosomal proteins. We have constructed a yeast strain in which one of the small subunit proteins, termed Mrp4, was tagged with S-peptide and used for affinity purification of… CONTINUE READING

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