Taenia solium: a cysteine protease secreted by metacestodes depletes human CD4 lymphocytes in vitro.

@article{Molinari2000TaeniaSA,
  title={Taenia solium: a cysteine protease secreted by metacestodes depletes human CD4 lymphocytes in vitro.},
  author={J L Molinari and Helen Mejia and Alexander C. White and Eduard Garrido and Ver{\'o}nica M Borgonio and Salman Baig and P. Tato},
  journal={Experimental parasitology},
  year={2000},
  volume={94 3},
  pages={133-42}
}
Excreted/secreted products from Taenia solium metacestodes cultured in vitro were analyzed for peptidase activity using peptide substrates Z-Phe-Arg-AFC, Arg-AFC, and Z-Gly-Gly-Arg-AFC and zymography studies. Specific inhibitor profiles revealed mainly cysteine and metalloprotease activities. Hydrolysis of substrate Z-Phe-Arg-AFC was augmented by the addition of L-cysteine and acid pH, consistent with cysteine protease activity. Cysteine protease activity was more prominent in supernatants from… CONTINUE READING