TUNEL analyses of bovine blastocysts after culture with EGF and IGF-I.

@article{Sirisathien2003TUNELAO,
  title={TUNEL analyses of bovine blastocysts after culture with EGF and IGF-I.},
  author={Saksiri Sirisathien and Benjamin G. Brackett},
  journal={Molecular reproduction and development},
  year={2003},
  volume={65 1},
  pages={51-6}
}
Experiments were carried out to investigate the beneficial effects of IGF-I or EGF on bovine embryo development in chemically defined embryo culture media and resultant incidences of nuclear DNA fragmentation as an indication of embryo quality. Presumptive IVF zygotes were randomly cultured in either control (with no added growth factor) or treatment groups, i.e., with 50 ng/ml IGF-I (experiment 1) or 5 ng/ml EGF (experiment 2). IGF-I supplemented to culture media significantly improved… CONTINUE READING