TTAGG Telomeric Repeats in Chromosomes of Some Insects and Other Arthropods

  title={TTAGG Telomeric Repeats in Chromosomes of Some Insects and Other Arthropods},
  author={Ken Sahara and Franti{\vs}ek Marec and W. Traut},
  journal={Chromosome Research},
We studied the occurrence of the TTAGG telomere repeats by fluorescence in-situ hybridization (FISH) and Southern hybridization in ten insect species and two other arthropods. (TTAGG)n-containing telomeres were found in three Lepidoptera species, the silkworm Bombyx mori (in which the telomeric sequence was recently discovered), the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella, in one species of Hymenoptera, the honey bee Apis mellifera, in one species of Coleoptera, the… 

Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization.

It is suggested that parasitic Acanthocephala have evolved yet unknown telomeric repeat motifs or different mechanisms of telomere maintenance.

Phylogenetic distribution of TTAGG telomeric repeats in insects.

The repeated losses of TTAGG in different branches of the insect phylogenetic tree and, in particular, in the most successful lineage of insect evolution, the Endopterygota, suggest a backup mechanism in the genome of insects that enabled them frequent evolutionary changes in telomere composition.

The presence of the ancestral insect telomeric motif in kissing bugs (Triatominae) rules out the hypothesis of its loss in evolutionarily advanced Heteroptera (Cimicomorpha)

Next-generation sequencing data analysis on Triatoma infestans Klug, 1834 revealed the presence of the ancestral insect (TTAGG)n telomeric motif in its genome, contradicting the currently accepted hypothesis that evolutionarily recent heteropterans lack this ancestral insect telomersic sequence.

Comparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae): is the insect canonical sequence conserved?

FISH analyses revealed the telomeric regions in all the chromosomes of the species studied in the present work were marked by the (TTAGG)6 sequence, reinforcing the premise that Formicidae presents high homology between their genera for the presence of the canonical sequence (TT AGG)n.

Are the TTAGG and TTAGGG telomeric repeats phylogenetically conserved in aculeate Hymenoptera?

It is suggested that the (TTAGG)n sequence was putatively lost in the ancestor of Apocrita with at least two subsequent independent regains (in Formicidae and Apidae) and the number of hymenopteran families lacking this telomeric sequence to 13 out of the 15 tested families so far.

First evidence for (TTAGG)n telomeric sequence and sex chromosome post-reduction in Coleorrhyncha (Insecta, Hemiptera)

It is shown that Peloridium pomponorum displays 2n = 31 (30A + X) in males, the classical insect (TTAGG)n telomere organization and sex chromosome post-reduction during spermatocyte meiosis.

Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae).

The leafhopper genus Alebra Fieber, 1872 comprises a complex of morphologically similar species that have a holokinetic type of chromosomes, as in other hemipterans and speciation in Alebra has apparently not involved significant karyotypic changes.

TCAGG, an alternative telomeric sequence in insects

Study of the distribution of telomeric DNAs in 30 coleopteran beetles showed that arrays built of a TCAGG repeat substitute for (TTAGG)n sequences in all tested species within the superfamily Tenebrionoidea, and provided the experimental evidence that (TCAGg)n repeats represent the terminal sequences on all chromosomes of the model species Tribolium castaneum.

Detection of telomeric sequences and ribosomal RNA genes in holokinetic chromosomes of five jumping plant-lice species: First data on the superfamily Psylloidea (Hemiptera: Sternorrhyncha)

It is found that the chromosomes of all studied species contain the insect-type telomere motif, (TTAGG)n, and is speculated, based on the obtained FISH markers, on the mechanisms of karyotype evolution of psylloid species differing in chromosome numbers and sex chromosome systems.



Identification of a pentanucleotide telomeric sequence, (TTAGG)n, in the silkworm Bombyx mori and in other insects

The Bombyx type of telomere sequence, (TTAGG)n, hybridized to DNAs from 8 of 11 orders of insect species tested but not to vertebrate DNAs, suggesting that this TTAGG repetitive sequence is conserved widely among insects.

Telomere sequence localization and karyotype evolution in higher plants

Data for chromosomal localization of theArabidopsis-type of telomeric sequence repeats (TTTAGGG)n are compiled for 44 species belonging to 14 families of angiosperms, gymnosperms and bryophytes, and it is inferred that they represent the basic telomere sequence of higher plant phyla.

Characterization of honeybee (Apis mellifera L.) chromosomes using repetitive DNA probes and fluorescence in situ hybridization.

This is the first report of an unambiguous identification of the chromosomal set of the honeybee, since classical banding techniques failed to yield clear patterns for identification.

FISH analysis of the telomere sequences of bulldog ants (Myrmecia: Formicidae)

Chromosomes from several species of ants from the genus Myrmecia were hybridized with deoxyoligomer probes of either (T2AG2)7, the putative insect telomere repeat sequence, or (T1AG3)3, the vertebrate telomeres repeat sequence to speculate on the origin of the new telomees as the chromosome numbers increased.

A family of complex tandem DNA repeats in the telomeres of Chironomus pallidivittatus

It is concluded that subfamilies are differentially distributed between and within telomeres and are likely to serve different functions in Chironomus pallidivittatus.

Chromosome end elongation by recombination in the mosquito Anopheles gambiae

Ch Chromosome end regression and extension were studied in a medically important mosquito, the malaria vector Anopheles gambiae, to determine how this dipteran insect maintains its chromosome ends.

Complex telomere-associated repeat units in members of the genus Chironomus evolve from sequences similar to simple telomeric repeats

It is proposed that the present-day Chironomus tentans unit has evolved from telomeric, simple sequence, asymmetric DNA from which it has retained some original sequence features and possibly functions.

Conservation of the human telomere sequence (TTAGGG)n among vertebrates.

The conservation of the (TTAGGG)n sequence and its telomeric location, in species thought to share a common ancestor over 400 million years ago, strongly suggest that this sequence is the functional vertebrate telomere.

Molecular differentiation of sex chromosomes probed by comparative genomic hybridization

Comparative genomic hybridization was used to identify and probe sex chromosomes in several XY and WZ systems and shows that W chromosomes in these species are molecularly well differentiated from the Z chromosomes.

Terminal long tandem repeats in chromosomes form Chironomus pallidivittatus

After adding synthetic oligonucleotide tails to DNA extracted from the microdissected right end of the fourth chromosome, it is demonstrated that the blocks of repeats were tailed at only one end, the chromosome terminus, the interior of the arrays being unavailable for tailing.