TSWV resistance in DH lines of tobacco (Nicotiana tabacum L.) obtained from a hybrid between ‘Polalta’ and ‘Wiślica’

  title={TSWV resistance in DH lines of tobacco (Nicotiana tabacum L.) obtained from a hybrid between ‘Polalta’ and ‘Wiślica’},
  author={Dorota Laskowska and Apoloniusz Berbe{\'c}},
  journal={Plant Breeding},
With 2 tables Abstract Nicotiana tabacum cv. ‘Polalta’ is reported as having a hypersensitive type of Tomato spotted wilt virus (TSWV) resistance. In ‘Polalta’ hybrids with susceptible cultivars, morphological deformations (thickened and ribbon-shaped leaves, irregular venation, tumours) appear. Acceptable DH lines derived from hybrids of ‘Polalta’ with ‘Wiślica’ were obtained by a method of in vitro culture. Those DH lines were evaluated for TSWV resistance. Tests for resistance were… 
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A survey of Nicotiana germplasm for resistance to Tomato spotted wilt virus (TSWV)
The reaction to Tomato spotted wilt virus (TSWV) was evaluated in 94 accessions of Nicotiana, originating from the Institute of Soil Science and Plant Cultivation tobacco germplasm collection in Puławy, Poland and demonstrated the presence of SCAR markers linked to the resistance gene.
Effectiveness of combining resistance to Thielaviopsis basicola and Tomato spotted wilt virus in haploid tobacco genotypes
The results indicate that small proportion of TSWV-resistant haploids is probably due to the influence of deleterious genes flanking the resistance factor that reduced vitality of gametophytes.
Resistance Response of the Recently Discovered Species Nicotiana mutabilis to Potato virus Y (PVY) and Tomato spotted wilt virus (TSWV) Compared to Other Sources of Resistance
This species extends the genetic variation in the genus Nicotiana and, because of its tolerance to PVY and partial resistance to TSWV, it may be a potential source of resistance to these viruses.
Morphological and chemical characteristics of doubled haploids of flue-cured tobacco combining resistance to Thielaviopsis basicola and TSWV
Biological tests and field performance of DH revealed potential to overcome the negative effect of coupling between the RSTV-al gene and genes responsible for the morphological deformations, which might be explained by a recombination during microsporogenesis in F1, however the influence of ‘Polalta’-derived RTSW- al gene cannot be excluded.
The Use of Nicotiana Species in Tobacco Improvement
This review examines how and to what extent natural germplasm resources have been utilized in hybridization and introgression experiments over the past century, and focuses on individual Nicotiana species involved in interspecific hybrids with cultivated tobacco produced by sexual and asexual methods.
Effectiveness of combining va-mediated resistance with a lettuce mosaic virus coat protein gene and a potato virus Y polymerase RNA gene in protection of tobacco hybrids from Potato virus Y
The most effective combination of va factor and transgene LMV CP was observed for VAM x MN 944 LMV and Wiślica x MN 900 LMV because a complete resistance was maintained in subsequent generations in the case of these hybrids.
Agronomic characteristics of transgenic tobacco doubled haploids resistant to Potato virus Y
The doubled haploids (DH) lines characterized by the most satisfying agronomic traits may be valuable initial material in further breeding, and in comparison to parental cultivars as regards some traits.


Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense ‘PI 159236’
Segregation ratios strongly indicated that the resistant response to TSWV fits a single-dominant gene model, and the resistance governed by the Tsw gene was not effective against isolates belonging to tomato chlorotic spot virus and groundnut ring spot virus, two previously described Tospovirus species.
Inheritance of a gene for resistance to tomato spotted wilt virus (TSWV) from Lycopersicon peruvianum Mill
The inheritance of TSWV resistance was found to be a single dominant gene, which indicates a 98.7% penetrance of the resistance gene in plants infected four months after the inoculations.
AFLP and SCAR Markers Linked to Tomato Spotted Wilt Virus Resistance in Tobacco
By selecting for resistant plants with an improved plant type and marker profile in a BC 3 population, four plants were identified that were missing 1 to 14 of the AFLP markers associated with the alien chromatin, demonstrating that rare recombination events can be identified.
Movement and distribution of tomato spotted wilt virus in resistant and susceptible accessions of Capsicum spp.
The DTB technique showed that viral movement within the inoculated leaf is restricted in the resistant plants, and detection of weak ELISA positives in inocul...
Cytology and fertility of viable hybrids of Nicotiana tabacum L. cv. TB-566 with N. alata Link et Otto.
Transfer programs based on the introgression of entire linkage groups (sexual and somatic hybridization) seem to be of little use in the case of N. tabacum because of a barrier caused by genic imbalance rather than irregularities of meiotic divisions.
Tomato spotted wilt virus is considered as the most widespread and to have the largest host-range and the availability of an up-to-date list of TSWV host plants is a potentially useful reference for researchers and farmers.
Haploid Plants from Pollen Grains
A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.
The method enabled the highly sensitive detection of a number of morphologically different viruses in purified preparations and in unclarified extracts of herbaceous hosts and of infected crop plants.