TSSC1 is novel component of the endosomal retrieval machinery

@article{Gershlick2016TSSC1IN,
  title={TSSC1 is novel component of the endosomal retrieval machinery},
  author={David C. Gershlick and Christina E. M. Schindler and Yu Chen and Juan S. Bonifacino},
  journal={Molecular Biology of the Cell},
  year={2016},
  volume={27},
  pages={2867 - 2878}
}
A previously uncharacterized WD40 domain–containing protein named TSSC1 is shown to interact with the GARP and EARP tethering complexes, promoting retrograde transport of Shiga toxin from endosomes to the TGN, as well as recycling internalized transferrin from endosomes to the plasma membrane. 

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References

SHOWING 1-10 OF 63 REFERENCES
Two novel WD40 domain–containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway
TLDR
Two novel WD40 domain proteins, Ere1 and Ere2 (endosomal recycling proteins), are found to mediate cargo-specific recognition by the retromer pathway.
Ang2/Fat-Free Is a Conserved Subunit of the Golgi-associated Retrograde Protein Complex
TLDR
The protein Ang2 is shown to be a conserved component of the Golgi-associated Retrograde Protein (GARP) complex in higher eukaryotes, contributing to the maintenance of acid hydrolase sorting, lysosome function and autophagy.
EARP, a multisubunit tethering complex involved in endocytic recycling
TLDR
A tethering complex named endosome-associated recycling protein (EARP) is described that is structurally related to the previously described Golgi-associated retrograde protein (GARP) complex, but EARP contains an uncharacterized protein, syndetin, in place of the Vps54 subunit of GARP.
Retrograde transport from endosomes to the trans-Golgi network
TLDR
A central role for a 'tubular endosomal network' in sorting to recycling pathways that lead not only to the trans-Golgi network but also to different plasma-membrane domains and to specialized storage vesicles is proposed.
Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform
TLDR
This study shows that membrane exchange between the early endocytic and the biosynthetic/secretory pathways involves specific components of the Rab and SNARE machinery, and suggests that retrograde transport between early/recycling endosomes and the endoplasmic reticulum is critically dependent on the sequential action of two members of therab6 subfamily.
Structural Analysis of the Interaction Between the SNARE Tlg1 and Vps51
TLDR
The structure of the N‐terminal domain of Tlg1 bound to a peptide from the N terminus of Vps51 is determined, and it is found that this particular interaction cannot be crucial to the process of vesicle docking or fusion.
Endocytic recycling
TLDR
After endocytosis, most membrane proteins and lipids return to the cell surface, but some membrane components are delivered to late endosomes or the Golgi, where they are sorted and recycled.
Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
Caenorhabditis elegans SNAP-29 is required for the proper morphology and functions of the Golgi and endosomes and general exocytosis.
Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells
TLDR
It is shown that expression of a dominant-negative construct with a point mutation near the EH domain results in redistribution of the endocytic recycling compartment and slowing down of transferrin receptor recycling.
...
...