TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat.

@article{Nealen2003TRPM8MI,
  title={TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat.},
  author={Michele L. Nealen and Michael S. Gold and Paul D. Thut and Michael J. Caterina},
  journal={Journal of neurophysiology},
  year={2003},
  volume={90 1},
  pages={
          515-20
        }
}
Recent electrophysiological studies of cultured dorsal root and trigeminal ganglion neurons have suggested that multiple ionic mechanisms underlie the peripheral detection of cold temperatures. Several candidate "cold receptors," all of them ion channel proteins, have been implicated in this process. One of the most promising candidates is TRPM8, a nonselective cationic channel expressed in a subpopulation of sensory neurons that is activated both by decreases in temperature and the cooling… 

Figures and Tables from this paper

The contribution of TRPM8 channels to cold sensing in mammalian neurones

TLDR
It is directly demonstrated that expression of TRPM8 in mammalian neurones induces cold sensing, albeit at lower temperatures than native TR PM8‐expressing neurones, suggesting the presence of additional modulatory mechanisms in the cold response of sensory neurones.

Variable Threshold of Trigeminal Cold-Thermosensitive Neurons Is Determined by a Balance between TRPM8 and Kv1 Potassium Channels

TLDR
It is suggested that innocuous cooling sensations and cold discomfort are signaled by specific low- and high-threshold cold thermoreceptor neurons, differing primarily in their relative expression of two ion channels having antagonistic effects on neuronal excitability.

Two populations of cold‐sensitive neurons in rat dorsal root ganglia and their modulation by nerve growth factor

TLDR
A picture is supported in which TRPM8 is the major player in detecting gentle cooling, while TRPA1 does not seem to be involved in cold sensing by MI neurons, at least in the temperature range between 32 and 12 °C.

Chemical and cold sensitivity of two distinct populations of TRPM8-expressing somatosensory neurons.

TLDR
Using acutely dissociated rat dorsal root ganglion neurons without culture, it is shown that TRPM8 receptors are expressed on two distinct classes of somatosensory neurons, one of which is sensitive to menthol and features nonnociceptive neuron properties, and the other has characteristics of nocICEptive neurons including capsaicin-sensitive, ATP- sensitive, prolonged acid response, and expression of bothTTX-sensitive and TTX-resistant sodium channels.

Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with aδ/c‐fibers and colocalization with trk receptors

TLDR
Heterogeneity of TRPM8 and TRPA1 expression by subpopulations of primary afferent neurons, which may result in the difference of cold‐sensitive primary afferential neurons in sensitivity to chemicals such as menthol and capsaicin and nerve growth factor, is suggested.

Sex differences in mouse Transient Receptor Potential Cation Channel, Subfamily M, Member 8 expressing trigeminal ganglion neurons

TLDR
Results suggest that sex differences in the functioning of Ih and Ik in TRPM8 expressing primary afferent neurons of male and female mice likely leads to greater sensitivity of female mice to the cool temperature.

Diversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 Neurons

TLDR
Results further demonstrate that the peripheral neural circuitry of cold sensing is cellularly and anatomically complex, yet suggests that cold fibers, caused by the diverse neuronal context of TRPM8 expression, use a single molecular sensor to convey a wide range of cold sensations.

Sensory discrimination between innocuous and noxious cold by TRPM8-expressing DRG neurons of rats

TLDR
TTXs/TRPM8 neurons and TTXr/TR PM8 neurons were shown to fire action potentials at innocuous and noxious cold temperatures respectively, demonstrating sensory discrimination between innocuous andNoxious cold by the two subpopulations of cold-sensing DRG neurons.

Contribution of TRPM8 Channels to Cold Transduction in Primary Sensory Neurons and Peripheral Nerve Terminals

TLDR
The minor impairment in the ability to transduce cold stimuli by peripheral corneal thermoreceptors during TRPM8 blockade unveils an overlapping functional role for various thermosensitive mechanisms in these nerve terminals.
...

References

SHOWING 1-10 OF 33 REFERENCES

Specificity of cold thermotransduction is determined by differential ionic channel expression

TLDR
This work identifies a population of cold-sensitive cultured mouse trigeminal ganglion neurons with a unique set of biophysical properties, and shows that cooling closes a background K+ channel, causing depolarization and firing that is limited by the slower reduction of a cationic inward current (Ih).

Identification of a cold receptor reveals a general role for TRP channels in thermosensation

TLDR
These findings, together with the previous identification of the heat-sensitive channels VR1 and VRL-1, demonstrate that TRP channels detect temperatures over a wide range and are the principal sensors of thermal stimuli in the mammalian peripheral nervous system.

A TRP Channel that Senses Cold Stimuli and Menthol

TRPV3 is a calcium-permeable temperature-sensitive cation channel

TLDR
It is proposed that hTRPV3 is thermosensitive in the physiological range of temperatures between TRPM8 and TRPV1.

Ion channels activated by cold and menthol in cultured rat dorsal root ganglion neurones

The expression of P2X3 purinoreceptors in sensory neurons: effects of axotomy and glial-derived neurotrophic factor.

TLDR
Glial cell line-derived neurotrophic factor (GDNF), delivered intrathecally, completely reversed axotomy-induced down-regulation of the P2X3 receptor and it is concluded that P2x3 receptors are normally expressed in nociceptive primary sensory neurons, predominantly the nonpeptidergic nocICEptors.

DEG/ENaC ion channels involved in sensory transduction are modulated by cold temperature

TLDR
The finding that cold temperature modulates DEG/ENaC channel function may provide a molecular explanation for the widely recognized ability of temperature to modify taste sensation and mechanosensation.

The Expression of P2X3Purinoreceptors in Sensory Neurons: Effects of Axotomy and Glial-Derived Neurotrophic Factor

TLDR
Glial cell line-derived neurotrophic factor (GDNF), delivered intrathecally, completely reversed axotomy-induced down-regulation of the P2X3 receptor and it is concluded that P2x3 receptors are normally expressed in nociceptive primary sensory neurons, predominantly the nonpeptidergic nocICEptors.