TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-{kappa}B activation.

@article{He2004TRAF3FH,
  title={TRAF3 forms heterotrimers with TRAF2 and modulates its ability to mediate NF-\{kappa\}B activation.},
  author={Liusheng He and Amrie C. Grammer and Xiaoli Wu and Peter E Lipsky},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 53},
  pages={55855-65}
}
FRET experiments utilizing confocal microscopy or flow cytometry assessed homo- and heterotrimeric association of human tumor necrosis factor receptor-associated factors (TRAF) in living cells. Following transfection of HeLa cells with plasmids expressing CFP- or YFP-TRAF fusion proteins, constitutive homotypic association of TRAF2, -3, and -5 was observed, as well as heterotypic association of TRAF1-TRAF2 and TRAF3-TRAF5. A novel heterotypic association between TRAF2 and -3 was detected and… CONTINUE READING