TOAC spin labels in the backbone of alamethicin: EPR studies in lipid membranes.

  title={TOAC spin labels in the backbone of alamethicin: EPR studies in lipid membranes.},
  author={Derek Marsh and Micha Jost and Cristina Peggion and C. Toniolo},
  journal={Biophysical journal},
  volume={92 2},
Alamethicin is a 19-amino-acid residue hydrophobic peptide that produces voltage-dependent ion channels in membranes. Analogues of the Glu(OMe)(7,18,19) variant of alamethicin F50/5 that are rigidly spin-labeled in the peptide backbone have been synthesized by replacing residue 1, 8, or 16 with 2,2,6,6-tetramethyl-piperidine-1-oxyl-4-amino-4-carboxyl (TOAC), a helicogenic nitroxyl amino acid. Conventional electron paramagnetic resonance spectra are used to determine the insertion and… CONTINUE READING
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