TNF-alpha suppresses dendritic cell death and the production of reactive oxygen intermediates induced by plasma withdrawal.

@article{Um2004TNFalphaSD,
  title={TNF-alpha suppresses dendritic cell death and the production of reactive oxygen intermediates induced by plasma withdrawal.},
  author={Hong-Duck Um and Young-hun Cho and Do Kyun Kim and Jong-Ran Shin and Yung-Jae Lee and Kwang-Sung Choi and J Kang and M Lee},
  journal={Experimental dermatology},
  year={2004},
  volume={13 5},
  pages={282-8}
}
Mature dendritic cells (DCs) were generated by culturing human peripheral blood monocytes for 7 days and, then, treating them with a cytokine cocktail for 2 days. The viability of the mature DCs (Day 9) obtained was approximately 60-70%, and this gradually declined when they were recultured in X-VIVO 15 media containing 2% human plasma (40% viability after… CONTINUE READING