TNF-α and LPS activate angiogenesis via VEGF and SIRT1 signalling in human dental pulp cells.

@article{Shin2015TNFAL,
  title={TNF-α and LPS activate angiogenesis via VEGF and SIRT1 signalling in human dental pulp cells.},
  author={M R Shin and S K Kang and Y S Kim and S Y Lee and Sungok Hong and Eun-Cheol Kim},
  journal={International endodontic journal},
  year={2015},
  volume={48 7},
  pages={705-16}
}
AIM To assess whether SIRT1 and VEGF are responsible for tumour necrosis factor-α (TNF-α) and lipopolysaccharide (LPS)-induced angiogenesis and to examine the molecular mechanism(s) of action in human dental pulp cells (HDPCs). METHODOLOGY Immortalized HDPCs obtained from Prof. Takashi Takata (Hiroshima University, Japan) were treated with LPS (1 μg mL(-1) ) and TNF-α (10 ng mL(-1) ) for 24 h. mRNA and protein levels were examined by RT-PCR and Western blotting, respectively. Migration and… CONTINUE READING