TNFα contributes for attenuating both Y397FAK and Y416Src phosphorylations in osteoblasts.

@article{Cavagis2014TNFCF,
  title={TNFα contributes for attenuating both Y397FAK and Y416Src phosphorylations in osteoblasts.},
  author={Adm Cavagis and Er Takamori and Jm Granjeiro and Rc Oliveira and Cv Ferreira and Mp Peppelenbosch and Wf Zambuzzi},
  journal={Oral diseases},
  year={2014},
  volume={20 8},
  pages={780-6}
}
OBJECTIVE Our poor understanding of how inflammatory mediators can affect osteoblast behavior led us to investigate the tumor necrosis factor (TNF)α-induced focal adhesion kinase (FAK) and Src phosphorylation. MATERIAL AND METHODS MC3T3-E1 pre-osteoblast cells were harvested at specific time points after either TNFα treatment or RAW267 stimulated conditioned medium, and thereafter cell extracts were prepared for Immunoblotting assay. ELISA detected TNFα content at conditioned medium. Tumor… CONTINUE READING