TLR4/NF-κB signaling pathway-mediated and oxLDL-induced up-regulation of LOX-1, MCP-1, and VCAM-1 expressions in human umbilical vein endothelial cells.

  title={TLR4/NF-$\kappa$B signaling pathway-mediated and oxLDL-induced up-regulation of LOX-1, MCP-1, and VCAM-1 expressions in human umbilical vein endothelial cells.},
  author={Y. Feng and Z. R. Cai and Y L Tang and G Hu and J. Lu and D H He and S Z Wang},
  journal={Genetics and molecular research : GMR},
  volume={13 1},
  • Y. FengZ. Cai S. Wang
  • Published 28 January 2014
  • Biology
  • Genetics and molecular research : GMR
This study aimed to investigate the function and signaling pathway of Toll-like receptor 4 (TLR4) in oxidized low-density lipoprotein (oxLDL)-induced up-regulated expressions of oxidized LDL receptor 1 (LOX-1), monocyte chemoattractant protein 1 (MCP-1), and vascular cell adhesion molecule 1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with different oxLDL concentrations (0, 20, 40, 60, and 80 μg/mL) for 24 and 48 h. The influence of oxLDL on TLR4, LOX-1… 

Novel function of fluvastatin in attenuating oxidized low-density lipoprotein-induced endothelial cell ferroptosis in a glutathione peroxidase4- and cystine-glutamate antiporter-dependent manner

The present study was the first to discover that fluvastatin may protect endothelial cells from ox-LDL-induced ferroptosis and dysfunction, and knockdown of GPx4 and xCT expression blunted the protective effects of fluVastatin on ox- LDL-treated endothelium cells.

DFMG attenuates the activation of macrophages induced by co-culture with LPC-injured HUVE-12 cells via the TLR4/MyD88/NF-κB signaling pathway

DFMG attenuated the activation of MP induced by co-culture with LPC-injured HUVE-12 cells, mediated via inhibition of the TLR4/MyD88/NF-κB signaling pathway in H UVE- 12 cells.

LOX-1: Regulation, Signaling and Its Role in Atherosclerosis

Different mechanisms for regulation, signaling and the various effects of LOX-1 in contributing to atherosclerosis are focused on.

The role of the lectin-like oxLDL receptor (LOX-1) in traffic-generated air pollution exposure-mediated alteration of the brain microvasculature in Apolipoprotein (Apo) E knockout mice

Findings suggest that alterations in brain microvascular structure and integrity observed with MVE-exposure may be mediated, at least in part, via LOX-1 signaling.

MiR-126 promotes endothelial cell apoptosis by targeting PI 3 K / Akt in rats with lower limb arteriosclerosis obliterans

In rats with lower limb arteriosclerosis obliterans (ASO), miR-126 represses the PI3K/Akt signaling pathway to accelerate endothelial cell apoptosis and is proven via the Luciferase assay.

Apoptosis in HUVECs induced by microRNA-616-3p via X-linked inhibitor of apoptosis protein targeting

It is demonstrated that microRNA (miR)-616-3p can directly inhibit the expression of XIAP mRNA by targeting its 3'UTR which promoted apoptosis in HUVECs.



Antisense to LOX-1 inhibits oxidized LDL-mediated upregulation of monocyte chemoattractant protein-1 and monocyte adhesion to human coronary artery endothelial cells.

It is suggested that LOX-1 is a key factor in ox-LDL-mediated monocyte adhesion to HCAECs, and activation of mitogen-activated protein kinase (MAPK) may play a critical role in signal transduction inOx-LDl-mediated alteration in MCP-1 expression.

Toll-Like Receptor 4–Dependent and –Independent Cytokine Secretion Induced by Minimally Oxidized Low-Density Lipoprotein in Macrophages

In macrophages, mmLDL activates TLR4-dependent and -independent signaling pathways, resulting in secretion of proinflammatory cytokines, providing new insights into the inflammatory origins of atherosclerosis.

The effects of ox-LDL in human atherosclerosis may be mediated in part via the toll-like receptor 4 pathway

Inhibition of TLR4 expression may downregulate the NF-κ B activity and secretions of MCP-1 and IL-8 in monocytes due to oxidized LDL, resulting in the alleviation of the progress of atherosclerosis.

Enhanced Levels of Oxidized Low-Density Lipoprotein Prime Monocytes to Cytokine Overproduction via Upregulation of CD14 and Toll-Like Receptor 4 in Unstable Angina

In UA patients oxLDL may contribute to monocyte overproduction of some cytokines by upregulating CD14 and TLR-4 expression.

Ligand specificity of LOX-1, a novel endothelial receptor for oxidized low density lipoprotein.

Endothelial dysfunction, or activation, elicited by oxidized low density lipoprotein (Ox-LDL) and its lipid constituents has been shown to play a key role in the pathogenesis of atherosclerosis. We

Atorvastatin downregulates BMP-2 expression induced by oxidized low-density lipoprotein in human umbilical vein endothelial cells.

Ox-LDL treatment significantly increased BMP-2 expression, which is associated with NF-kappaB activation, but BMP -2 expression was suppressed by treatment with PDTC or atorvastatin, and the increase in MDA levels and decrease in activities of total SOD caused by ox- LDL treatment were reversed by the treatment of PDTC and atorVastatin.

Lack of Toll-like receptor 4 or myeloid differentiation factor 88 reduces atherosclerosis and alters plaque phenotype in mice deficient in apolipoprotein E.

  • K. MichelsenM. Wong M. Arditi
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 2004
An important role for TLR4 and MyD88 signaling in atherosclerosis in a hypercholesterolemic mouse model is suggested, providing a pathophysiologic link between innate immunity, inflammation, and atherogenesis.

Enhanced expression of endothelial oxidized low-density lipoprotein receptor (LOX-1) in hypertensive rats.

Results indicated that LOX-1 expression in the aorta and vein was upregulated in hypertensive rats, which may be involved in the impaired endothelium-dependent vasodilation in these rats.