TIPI: TEV protease-mediated induction of protein instability.

@article{Taxis2012TIPITP,
  title={TIPI: TEV protease-mediated induction of protein instability.},
  author={Christof Taxis and Michael Knop},
  journal={Methods in molecular biology},
  year={2012},
  volume={832},
  pages={611-26}
}
Reverse genetics approaches require methods to inactivate a specific protein. One possibility is to modify the target protein with a degradation signal (degron). Degrons are short, transferable sequences that confer protein instability. They target proteins for degradation either constitutively or after activation, e.g., by phosphorylation, presence of a binding partner, or conformational rearrangements in the substrate. In this chapter, we describe a synthetic way to activate a degron. It… CONTINUE READING
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