TILLING is an effective reverse genetics technique for Caenorhabditis elegans

@article{Gilchrist2006TILLINGIA,
  title={TILLING is an effective reverse genetics technique for Caenorhabditis elegans},
  author={Erin J. Gilchrist and Nigel J. O'Neil and A. M. Rose and Monique C. Zetka and George W Haughn},
  journal={BMC Genomics},
  year={2006},
  volume={7},
  pages={262 - 262}
}
TILLING (T argeting I nduced L ocal L esions i n G enomes) is a reverse genetic technique based on the use of a mismatch-specific enzyme that identifies mutations in a target gene through heteroduplex analysis. We tested this technique in Caenorhabditis elegans, a model organism in which genomics tools have been well developed, but limitations in reverse genetics have restricted the number of heritable mutations that have been identified. To determine whether TILLING represents an effective… CONTINUE READING
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