THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

@article{Reynolds1963THEUO,
  title={THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY},
  author={Edward S. Reynolds},
  journal={The Journal of Cell Biology},
  year={1963},
  volume={17},
  pages={208 - 212}
}
  • E. Reynolds
  • Published 1 April 1963
  • Biology
  • The Journal of Cell Biology
Aqueous solutions of lead salts (1, 2) and saturated solutions of lead hydroxide (1) have been used as stains to enhance the electron-scattering properties of components of biological materials examined in the electron microscope. Saturated solutions of lead hydroxide (1), while staining more intensely than either lead acetate or monobasic lead acetate (l , 2), form insoluble lead carbonate upon exposure to air. The avoidance of such precipitates which contaminate surfaces of sections during… 

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