THE TUBE METHOD OF MEASURING THE GROWTH RATE OF NEUROSPORA

@article{Ryan1943THETM,
  title={THE TUBE METHOD OF MEASURING THE GROWTH RATE OF NEUROSPORA},
  author={Francis J. Ryan and George Wells Beadle and Edward L. Tatum},
  journal={American Journal of Botany},
  year={1943},
  volume={30},
  pages={784-799}
}
IN CONNECTION with investigations on the biochemical genetics of the ascomycete Neurospora, a technique has been developed for studying growth by measuring the progression of mycelial frontiers within horizontal glass tubes half filled with suitable solid culture media. This method is the equivalent of measuring the increase in radius of a circular colony, a method used by many workers in studying growth of fungi, bacteria, and tissue cultures. The tube method is particularly useful in studying… 
GROWTH CHARACTERISTICS OF FUNGI AND ACTINOMYCETES
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The present investigation was undertaken to test the validity of Emerson's findings and to use the data obtained to determine the growth characteristics of a number of filamentous microorganisms.
Observations on the tube method of measuring growth rate in Neurospora crassa.
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  • Biology, Medicine
    Journal of general microbiology
  • 1968
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The relationship between various parameters of growth of Neurospora crassa on solid media in tubes, including culture weight and linear growth rate, appears to obey a log law, enabling an inoculum doubling-time to be calculated.
Naturally occurring cytoplasmic and nuclear changes in continuously growing cultures of Neurospora crassa
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Although a permanent cessation of growth was not observed in Neurospora, the characteristics of stp-A and sto-B suggest that these variants are related to "vegetative death" in Aspergillus, and "senescence in Podosuora and other fungi".
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    Archiv für Mikrobiologie
  • 2004
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Aeration stimulates conidiation in growth-tube cultures, thereby allowing experiments which demonstrate that nearly all strains and species of Neurospora are capable of circadianConidiation, and increases the persistence of circadianconidiation suggesting that the damping of rhythmicity previously observed on certain media represents the cessation of conidiated, rather than the stopping of a timing mechanism.
A cytoplasmic variant of Neurospora crassa.
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Heterokaryotic transmission was demonstrated using a suspension of germinated conidia of pan al-I on the germling by means of a Pasteur pipette, and the mutant phenotype originally associated with one component was now associated with the second component, this was considered evidence for the extranuclear basis of the Mutant phenotype.
THE EFFECT OF TEMPERATURE ON THE PRODUCTION OF PERITHECIA BY NEUROSPORA CRASSA.
The wild-type strains of Neurospora crassa first isolated by Dodge, Abbott & Chilton were found to differ in the range of temperatures over which protoperithecia were produced; similar results were
Mutations during the Growth of Biochemical Mutants of Escherichia Coli.
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A RELATIONSHIP BETWEEN CELL WALL STRUCTURE AND COLONIAL GROWTH IN NEUROSPORA CRASSA
TLDR
Quantitative analysis has shown that a group of 4 colonial mutants which strongly resemble each other in morphology all have significantly less glucose and more glucosamnine per unit weight of cell wall than do their corresponding wild-type strains, which strongly suggest that a particular type of morphological aberration in Neurospora is associated with similar changes in cell wall composition.
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