THE MORPHOLOGY AND ACETYLCHOLINE CONTENT OF ISOLATED CEREBRAL CORTICAL SYNAPTIC VESICLES

@article{Whittaker1965THEMA,
  title={THE MORPHOLOGY AND ACETYLCHOLINE CONTENT OF ISOLATED CEREBRAL CORTICAL SYNAPTIC VESICLES},
  author={Victor P. Whittaker and Michael N. Sheridan},
  journal={Journal of Neurochemistry},
  year={1965},
  volume={12}
}
WHEN brain tissue is homogenized under carefully controlled conditions, a high proportion of the presynaptic nerve terminals are torn away from their attachments to form discrete particles for which the term ‘synaptosomes’ has recently been adopted (WHITTAKER, MICHAELSON and KIRKLAND, 1964). These particles may be separated from other subcellular brain particles by differential and density gradient centrifuging. They retain all the structural features of the nerve endings, together with their… 
SOME PROPERTIES OF SYNAPTIC MEMBRANES ISOLATED FROM THE CENTRAL NERVOUS SYSTEM *
TLDR
The purpose of this communication is to describe three homogeneous membrane fractions derived from three specific portions of the central neuron which the authors have succeeded in isolating from homogenates of brain tissue.
The morphology of the contact region of vertebrate synaptosomes
  • D. Jones
  • Biology
    Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
TLDR
The classification of synaptosomes into Types A and B is discussed, particular attention being paid to their possible relationship to excitatory and inhibitory synapses.
Thirty years of synaptosome research
SummaryDetached synapses (synaptosomes), first isolated by the author in 1958 and identified as such in 1960, are sealed presynaptic nerve terminals often with a portion of the target cell —
The Synaptic Vesicle
TLDR
The synaptic vesicles of the electromotor nerve terminals in the electric organ of Torpedo are larger than normal; these vesicle can be isolated relatively easily in bulk and high purity and have thus provided an important source material for most of the recent work on the biochemistry and biophysics of synaptic vesa.
THE SYNTHESIS AND STORAGE OF ACETYLCHOLINE IN THE STRIATUM *
  • A. Sattin
  • Biology
    Journal of neurochemistry
  • 1966
TLDR
By disrupting synaptosomes, homogenization in fluid hypotonic to plasma releases synaptic vesicles, and presumably the associated structural changes allow rapid enzymic destruction of extra-vesicular ACh, it cannot yet be ruled out that some or most of the labile bound ACh is also bound to the vesicle in the intact nerve ending and appears factitiously as the ‘labile fraction’ when nerve endings are disrupted.
Tissue fractionation methods in brain research.
The subcellular fractionation of the electric organ of Torpedo
TLDR
The acetylcholine-rich electric organ of Torpedo has been submitted to subcellular fractionation in an attempt to isolate nerve endings and synaptic vesicles derived from cholinergic neurones, but the tissue proved difficult to homogenize and few detached nerve endings were formed.
Isolation and Study of Synaptic Vesicles
TLDR
This chapter describes methods currently available and offers some comments on their applicability and interpretation.
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