THE MINIMUM VITAMIN REQUIREMENTS OF THE L AND HELA CELLS IN TISSUE CULTURE, THE PRODUCTION OF SPECIFIC VITAMIN DEFICIENCIES, AND THEIR CURE

@article{Eagle1955THEMV,
  title={THE MINIMUM VITAMIN REQUIREMENTS OF THE L AND HELA CELLS IN TISSUE CULTURE, THE PRODUCTION OF SPECIFIC VITAMIN DEFICIENCIES, AND THEIR CURE},
  author={H. Eagle},
  journal={The Journal of Experimental Medicine},
  year={1955},
  volume={102},
  pages={595 - 600}
}
  • H. Eagle
  • Published 1955
  • Biology, Medicine
  • The Journal of Experimental Medicine
Seven vitamins have to date proved essential for the survival and multiplication of a mouse fibroblast (strain L) and a human carcinoma cell (strain HeLa) in tissue culture: choline, folic acid, nicotinamide, pantothenic acid, pyridoxal, riboflavin, and thiamin. It was necessary to cultivate the cells for 5 to 15 days in a medium lacking the specific vitamin before the deficiency became apparent in the cessation of multiplication and the development of specific cytopathogenic effects. In their… Expand
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