THE ISOLATION OF CHOLINERGIC SYNAPTIC VESICLES FROM BOVINE SUPERIOR CERVICAL GANGLION AND ESTIMATION OF THEIR ACETYLCHOLINE CONTENT

@article{Wilson1973THEIO,
  title={THE ISOLATION OF CHOLINERGIC SYNAPTIC VESICLES FROM BOVINE SUPERIOR CERVICAL GANGLION AND ESTIMATION OF THEIR ACETYLCHOLINE CONTENT},
  author={William Stuart Wilson and Ronald A. Schulz and Jack R. Cooper},
  journal={Journal of Neurochemistry},
  year={1973},
  volume={20}
}
Abstract— A method is described for the isolation of relatively pure cholinergic synaptic vesicles by hypo‐osmotic shock and density gradient centrifugation of a synaptosome fraction prepared from bovine superior cervical ganglia. Vesicles from this source were found to sediment to a density equivalent to 0·3–0·41 M‐sucrose. The vesicle subfraction from the gradient had an acetylcholine (ACh) content of 4.4 nmol/mg of protein and were subject to leakage of ACh. By a ‘tagging’ technique, the… 
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34 Citations

34 Citations

Citation Type

Citation Type

Isolation of Cholinergic Synaptic Vesicles from the Myenteric Plexus of Guinea‐Pig Small Intestine
The acetylcholine‐rich myenteric plexus‐longitudinal muscle preparation of the guinea‐pig small intestine has been subjected to subcellular fractionation using modifications of both classical methods…
The preparation and characterization of synaptic vesicles of high purity
Adenosine triphosphate in cholinergic vesicles isolated from the electric organ of electrophorus electricus
The subcellular localization of noradrenaline and dopamine in bovine superior cervical ganglion.
The Synaptic Vesicle
TLDR
The synaptic vesicles of the electromotor nerve terminals in the electric organ of Torpedo are larger than normal; these vesicle can be isolated relatively easily in bulk and high purity and have thus provided an important source material for most of the recent work on the biochemistry and biophysics of synaptic vesa.
Subcellular fractionation of cat submandibular gland: Comparative studies on the distribution of acetylcholine and vasoactive intestinal polypeptide (VIP)
The heterogeneity of vesicular acetylcholine storage in cholinergic nerve terminals.
The electromotor system of Torpedo. A model cholinergic system.
TLDR
Studies of the protein composition of the vesicle membrane, especially when combined with similar information about the external presynaptic membrane, promise to throw new light on the molecular mechanism underlying vesicles exo-/endocytosis.
Acetylcholine, ATP, and Proteoglycan Are Common to Synaptic Vesicles Isolated from the Electric Organs of Electric Eel and Electric Catfish as Well as from Rat Diaphragm
TLDR
ACh, ATP, and proteoglycan are common molecular constituents of motor nerve terminal‐derived synaptic vesicles from Torpedo to rat and may play a specific role in the process of cholinergic signal transmission.
SEPARATION OF ACETYLCHOLINE AND CATECHOLAMINE CONTAINING SYNAPTIC VESICLES FROM BRAIN CORTEX
Abstract— Synaptic vesicles were prepared from guinea‐pig cerebral cortex on a continuous D2O‐H2O(1:1)‐sucrose gradient and purified in the presence of 1 mm‐EGTA by chromatography on columns of glass…
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References

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