THE ISOLATION OF CHOLINERGIC SYNAPTIC VESICLES FROM BOVINE SUPERIOR CERVICAL GANGLION AND ESTIMATION OF THEIR ACETYLCHOLINE CONTENT

@article{Wilson1973THEIO,
  title={THE ISOLATION OF CHOLINERGIC SYNAPTIC VESICLES FROM BOVINE SUPERIOR CERVICAL GANGLION AND ESTIMATION OF THEIR ACETYLCHOLINE CONTENT},
  author={W. S. Wilson and R. A. Schulz and J. Cooper},
  journal={Journal of Neurochemistry},
  year={1973},
  volume={20}
}
Abstract— A method is described for the isolation of relatively pure cholinergic synaptic vesicles by hypo‐osmotic shock and density gradient centrifugation of a synaptosome fraction prepared from bovine superior cervical ganglia. Vesicles from this source were found to sediment to a density equivalent to 0·3–0·41 M‐sucrose. The vesicle subfraction from the gradient had an acetylcholine (ACh) content of 4.4 nmol/mg of protein and were subject to leakage of ACh. By a ‘tagging’ technique, the… Expand
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  • 19
...
1
2
3
4
...

References

SHOWING 1-10 OF 26 REFERENCES
THE PREPARATION OF CHOLINERGIC SYNAPTOSOMES FROM BOVINE SUPERIOR CERVICAL GANGLIA
  • 26
BOUND ACETYLCHOLINE IN THE NERVE ENDING PARTICLES 1
  • 13
SOME FEATURES OF THE SUBMICROSCOPIC MORPHOLOGY OF SYNAPSES IN FROG AND EARTHWORM
  • 496
  • PDF
Choline acetyltransferase binding to and release from membranes.
  • F. Fonnum
  • Chemistry, Medicine
  • The Biochemical journal
  • 1968
  • 186
...
1
2
3
...