THE HYDROLYSIS OF CHITIN BY CONCENTRATED HYDROCHLORIC ACID, AND THE PREPARATION OF LOW-MOLECULAR-WEIGHT SUBSTRATES FOR LYSOZYME.

@article{Rupley1964THEHO,
  title={THE HYDROLYSIS OF CHITIN BY CONCENTRATED HYDROCHLORIC ACID, AND THE PREPARATION OF LOW-MOLECULAR-WEIGHT SUBSTRATES FOR LYSOZYME.},
  author={J. Rupley},
  journal={Biochimica et biophysica acta},
  year={1964},
  volume={83},
  pages={
          245-55
        }
}
  • J. Rupley
  • Published 1964
  • Chemistry, Medicine
  • Biochimica et biophysica acta
The hydrolysis of chitin in solution in strong HCl was studied over a range of acid concentration and temperature. The data suggest that the rate of hydrolysis governed by the structure of the chitin. A simple procedure for the isolation of the oligosaccharides obtained from chitin was developed, for use in the preparation of low-molecular-weight substrates for lysozyme (N-acetylmuramide glycanohydrolase, EC 3.2.1.17). Experiments studying the lysozyme-catalyzed hydrolysis of these… Expand
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