THE EFFECT OF MUTATION OF TWO FORMS OF PHOSPHOGLUCOMUTASE IN SACCHAROMYCES.

@article{Tsoi1964THEEO,
  title={THE EFFECT OF MUTATION OF TWO FORMS OF PHOSPHOGLUCOMUTASE IN SACCHAROMYCES.},
  author={A. N. Tsoi and Howard C. Douglas},
  journal={Biochimica et biophysica acta},
  year={1964},
  volume={92},
  pages={
          513-20
        }
}
The Saccharomyces haploid stock 8050B is a galactose fermenter which was found by chromatography on CM-cellulose and electrophoresis on agar gel to contain a major and a minor form of phosphoglucomutase (d-glucose-1,6-diphosphate:d-glucose-1-phosphate phosphotransferase, EC 2.7.5.1). Mutations in the Ga5 locus of this stock reduced the phosphoglucomutase activity in extracts 4–5-fold and rendered the cells unable to ferment galactose. The reduction in enzyme activity of the mutants was shown to… CONTINUE READING

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