THE DISTRIBUTION OF COLONY-FORMING CELLS AMONG SPLEEN COLONIES.

@article{Siminovitch1963THEDO,
  title={THE DISTRIBUTION OF COLONY-FORMING CELLS AMONG SPLEEN COLONIES.},
  author={L. Siminovitch and E. McCulloch and J. Till},
  journal={Journal of cellular and comparative physiology},
  year={1963},
  volume={62},
  pages={
          327-36
        }
}
Progenitor cells that are recognized by their ability to form colonies of descendants in the spleens of irradiated mice have the capacity for self-renewal. The distribution of new colony-forming cells per colony is extremely heterogeneous, indicating lax control of self-renewal. The capacities of colony-forming cells for self-renewal, for extensive proliferation, and for giving rise to differentiated descendants, fulfill three requirements for studies of stem cells. Thus, colony-forming cells… Expand
DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE.
TLDR
It was found that the radiation sensitivity of colony-forming ability increases in cells subjected to serial transplantation, which suggests that the self-renewal process does not necessarily result in an exact replication of the original colony-formation cells. Expand
The cellular composition of hemopoietic spleen colonies
TLDR
It was found that, 14 days after the initiation of spleen colonies, the distribution of these cell types among individual colonies was very heterogeneous, but that most colonies contained detectable numbers of erythroblasts, granulocytes and colony‐forming cells. Expand
Physical separation of hemopoietic stem cells from cells forming colonies in culture
TLDR
Mouse bone marrow cells in suspension were separated into a number of fractions on the basis of cell density by equilibrium density gradient centrifugation, or on the based of cell size by velocity sedimentation, to demonstrate that cells in some fractions formed more colonies in vivo than in the culture system. Expand
An antigenic difference between cells forming early and late haematopoietic spleen colonies (CFU-S)
TLDR
An antigenic difference between subpopulations of CFU-S forming early (day 8) and late (day 14) spleen colonies which has been used to purify multipotential haematopoietic stem cells from murine bone marrow is reported. Expand
PHYSICAL SEPARATION OF HEMOPOIETIC STEM CELLS DIFFERING IN THEIR CAPACITY FOR SELF-RENEWAL
TLDR
It may be concluded that at least part of the heterogeneity observed in the CFU content of individual spleen colonies arises from the composition of the initial cell suspension, probably from intrinsic differences between the stem cells themselves. Expand
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The target of CPA appears to constitute a separate compartment in the progenitor populations of granulocytic lineage, which is independent of those of other cell populations, namely pluripotent stem cells in vitro and GM-CFC in the spleen and bone marrow. Expand
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The results indicate that the substance(s) required for the growth and differentiation of “mast” cells can pass through agar. Expand
A cytological study of the capacity for differentiation of normal hemopoietic colony‐forming cells
TLDR
Support is provided for the view that colony forming hemopoietic stem cells are multipotent, and that differentiation along more than one pathway can occur during the formation of macroscopic splenic colonies. Expand
The self renewal probability of hemopoietic stem cells
TLDR
The results of both procedures indicate a stem cell self renewal probability p =0.62 ± 0.04, which does not change significantly between the sixth and the fourteenth day of colony development, and an extinction probability ω = 0.12. Expand
CFU-S in individual erythroid colonies derived in vitro from adult mouse marrow
TLDR
It is reported that cells capable of macroscopic spleen colony formation (CFU-S)6 are present in Macroscopic bursts and that on average two to three and up to five self-renewal divisions of CF U-S can occur during the first 9 d of burst growth. Expand
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