THE DISTRIBUTION AND PROPERTIES OF THE NONSPECIFIC N‐METHYLTRANSFERASE IN BRAIN

@article{Saavedra1973THEDA,
  title={THE DISTRIBUTION AND PROPERTIES OF THE NONSPECIFIC N‐METHYLTRANSFERASE IN BRAIN},
  author={Juan M. Saavedra and Joseph T. Coyle and Julius Axelrod},
  journal={Journal of Neurochemistry},
  year={1973},
  volume={20}
}
Abstract— The distribution and properties of a nonspecific N‐methyltransferase in the rat brain are described. The enzyme N‐methylates tryptamine and N‐methyltryptamine as well as β‐phenylethylamine, phenylethanolamine, tyramine and octopamine. The enzyme exhibits a pH optimum of 7·9 with phosphate buffer and has a Km for tryptamine of 28 μM. There are potent inhibitors to the enzyme that can be removed by dialysis. Enzymatic activity is present in the brains of a number of species including… 

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References

SHOWING 1-10 OF 17 REFERENCES

The enzymatic N-methylation of serotonin and other amines.

  • J. Axelrod
  • Biology, Chemistry
    The Journal of pharmacology and experimental therapeutics
  • 1962
The serotonin methylating enzyme can N-methylate a variety of normally occurring compounds such as dopamine, normetanephrine, metanephine, epinephrine, tyramine, and octopamine as well as a varietyof foreign compounds suchAs ephedrine, amphetamine, aniline, normorphine,normeperidine and nornicotine.

Indole(ethyl)amine N-methyltransferase in human brain.

Preliminary evidence gathered in laboratories from rats infused intraventricularly with bufotenin has suggested that this substance is at least as potent as its powerfully hallucinogenic 5-methoxy congener, and may not easily cross the blood-brain barrier.

Psychotomimetic N-Methylated Tryptamines: Formation in Brain in vivo and in vitro

An enzyme that converts tryptamine and N-methyl-tryptamine to N- methyl-and dimethyltryptamine was found to be present in rat and human brain.

Enzymatic conversion of norepinephrine to epinephrine by the brain.

The epinephrine-forming enzyme phenylethanolamine-N-methyl transferase (PNMT) can be demonstrated in various brain regions of the rat, cat, hen and turtle. The activity of the enzyme is greatest when

Indole(ethyl)amine N-Methyltransferase in the Brain

An enzyme which N-methylates various indole(ethyl)amine substrates was isolated from brain, separated from the pituitary and the pineal glands. It appeared localized in the supernatant and

REGIONAL STUDIES OF CATECHOLAMINES IN THE RAT BRAIN‐I

It is revealed that norepinephrine and dopamine are specifically localized in complex systems of neurons in the brain, a finding which lends support to the hypothesis that both amines may be neurotransmitters in the central nervous system.

Enzymatic Formation of Psychotomimetic Metabolites from Normally Occurring Compounds

An enzyme has been found that N-methylates serotonin and tryptamine to psychotomimetic metabolites, bufotenine and N,N-dimethyltryptamine. This enzyme is highly localized in the rabbit lung and also

Enzymatic Synthesis of Adrenaline in Mammalian Brain

It is found that adrenaline is synthesized in the brain through the uptake, metabolism and synthesis of adrenaline by mammalian brain.