THE DETERMINATION OF GLYCOGEN

@article{Good1933THEDO,
  title={THE DETERMINATION OF GLYCOGEN},
  author={Clarence A. Good and H. Kramer and Michael Somogyi},
  journal={Journal of Biological Chemistry},
  year={1933},
  volume={100},
  pages={485-491}
}
Bierry and Gruzewska (1, 2) were the first to eliminate the precipitation of glycogen. In their method the tissue (liver or muscle) is first hydrolyzed .with KOH, then autoclaved with acid; the fluid is treated with mercuric nitrate to remove nitrogenous substances, and in the resulting solution reducing matter is determined by Bertrand’s method. It is assumed that the reduction represents glucose derived from glycogen. Subsequent workers omitted the treatment with alkali. They removed… Expand

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