THE CIRCADIAN RHYTHM OF BIOLUMINESCENCE IN PYROCYSTIS IS NOT DUE TO DIFFERENCES IN THE AMOUNT OF LUCIFERASE: A COMPARATIVE STUDY OF THREE BIOLUMINESCENT MARINE DINOFLAGELLATES

@article{Knaust1998THECR,
  title={THE CIRCADIAN RHYTHM OF BIOLUMINESCENCE IN PYROCYSTIS IS NOT DUE TO DIFFERENCES IN THE AMOUNT OF LUCIFERASE: A COMPARATIVE STUDY OF THREE BIOLUMINESCENT MARINE DINOFLAGELLATES},
  author={Rosemarie Knaust and Thomas Urbig and Liming Li and Walter Taylor and John Woodland Hastings},
  journal={Journal of Phycology},
  year={1998},
  volume={34}
}
The biochemistry and circadian regulation of luminescence in two Pyrocystis species, P. lunula Hulburt and P. noctiluca Murray et Haeckel, were compared with a well‐studied species, Gonyaulax polyedra Stein. All exhibit circadian rhythms and all have similar luciferins and luciferases. However, the Pyrocystis species lack a second protein involved in the reaction in Gonyaulax, the luciferin (substrate) binding protein, which sequesters the luciferin at the cytoplasmic pH and releases it upon… Expand
New Perspectives Related to the Bioluminescent System in Dinoflagellates: Pyrocystis lunula, a Case Study
TLDR
A review of the literature in relation to the molecular players responsible for bioluminescence in dinoflagellates, with particular interest in P. lunula, and a phylogenetic analysis of the conservation of protein sequence, structure and evolutionary pattern of these key players. Expand
Diversity of the Luciferin Binding Protein Gene in Bioluminescent Dinoflagellates – Insights from a New Gene in Noctiluca scintillans and Sequences from Gonyaulacoid Genera
TLDR
Phylogenetic analyses showed that, in some species, the evolution of the luciferin binding protein gene was different from the organism's general phylogenies, highlighting the complex evolutionary history of dinoflagellate bioluminescence systems. Expand
Circadian Rhythms in Dinoflagellates: What Is the Purpose of Synthesis and Destruction of Proteins?
TLDR
It is posits that synthesis and destruction constitutes a cellular mechanism to conserve nitrogen in an environment where the resource is limiting. Expand
Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
TLDR
It is reported that the structural organization of the luciferase gene of Noctiluca scintillans is strikingly different from that of the seven luminous species previously characterized, all of which are photosynthetic. Expand
Circadian Changes in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Distribution Inside Individual Chloroplasts Can Account for the Rhythm in Dinoflagellate Carbon Fixation
TLDR
The rhythm in Rubisco distribution is causally related to the rhythm in carbon fixation and it is suggested that several mechanisms involving enzyme sequestration could account for the increase in the efficiency of carbon fixation. Expand
Cell ultrastructural changes correlate with circadian rhythms in Pyrocystis lunula (Pyrrophyta)
TLDR
The complete serial reconstruction of P. lunula cells during both day and night phases is reported, the first four dimensional study of any dinoflagellate. Expand
Effects of salinity, pH and temperature on the re-establishment of bioluminescence and copper or SDS toxicity in the marine dinoflagellate Pyrocystis lunula using bioluminescence as an endpoint.
TLDR
Re-establishment of bioluminescence showed a direct dependence on temperature, but only at 10 degrees C did temperature have an obvious effect on the toxicity of Cu in this bioassay. Expand
EFFECTS OF METALS AND ORGANIC CONTAMINANTS ON THE RECOVERY OF BIOLUMINESCENCE IN THE MARINE DINOFLAGELLATE PYROCYSTIS LUNULA (DINOPHYCEAE)1
TLDR
Investigation of the short‐term effects of metals and organic pollutants on the recovery of the bioluminescent potential in Pyrocystis lunula shows that it is sensitive to the reference contaminants tested and obtains highly reproducible results. Expand
Theoretical Study of Dinoflagellate Bioluminescence
TLDR
The density functional calculation is employed and results agree with the experimental observations and indicate the luciferin or its analogue, rather than oxyluciferin, is the bioluminophore of dinoflagellate bioluminescence. Expand
Involvement of actin and microtubules in regulation ofbioluminescence and translocation of chloroplasts in thedinoflagellate Pyrocystis lunula
TLDR
Results suggest that F-actin and microtubules are involved in the movements of chloroplasts and scintillons, and further experiments are needed to determine whether effects on bioluminescence were mediated by drug-induced failed translocations of scintsillons or chloroplast. Expand
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References

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CIRCADIAN REGULATION OF BIOLUMINESCENCE IN THE DINOFLAGELLATE PYROCYSTIS LUNULA 1
TLDR
The results indicate that the circadian regulation of luciferase activity differs in the two species, and the glow in P. lunula differs markedly from that in G. polyedra in that it may fluctuate 5–10‐fold in intensity within a time frame of seconds. Expand
The biological clock in Gonyaulax controls luciferase activity by regulating turnover.
TLDR
The purification and properties of the higher molecular weight unproteolyzed luciferase from both day and night phase cells are described, suggesting that theLuciferase is the same polypeptide in day and day extracts, and that such extracts contain different amounts of the enzyme. Expand
Dinoflagellate luciferases: purification of luciferases from Gonyaulax polyedra, Pyrocystis lunula, and Pyrocystis fusiformis.
TLDR
The pH dependence of the luciferase activities is represented by a symmetrical function with optimum near pH 7.4, and the flashing mechanism cannot be explained by means of a switch mechanism controlled by the pH. Expand
Dinoflagellate bioluminescence: A comparative study of invitro components
TLDR
In vitro bioluminescence components of the dinoflagellates Gonyaulax polyedra, G. tamarensis, Dissodinium lunula, and Pyrocystis noctiluca were studied and the observations that D. lunula and P. noCTiluca apparently lack LBP and have luciferases with low MW single chains require further clarification. Expand
Cloning, sequencing and expression of dinoflagellate luciferase DNA from a marine alga, Gonyaulax polyedra.
TLDR
This represents the first dinoflagellate Luciferase to be cloned and sequenced; its deduced amino acid sequence bears no significant homologies with that of any other luciferase, or any other sequence in the data base. Expand
Compartmentalization of algal bioluminescence: autofluorescence of bioluminescent particles in the dinoflagellate Gonyaulax as studied with image-intensified video microscopy and flow cytometry
TLDR
The endogenous fluorescence of the dinoflagellate alga Gonyaulax originates in vivo from discrete subcellular loci that are intrinsically fluorescent, and luciferin is localized within the cell at discrete loci from which the bioluminescence emanates. Expand
The polypeptide components of scintillons, the bioluminescence organelles of the dinoflagellate Gonyaulax polyedra.
  • M. Desjardins, D. Morse
  • Biology, Medicine
  • Biochemistry and cell biology = Biochimie et biologie cellulaire
  • 1993
Scintillons, the bioluminescence organelles of Gonyaulax polyedra, were purified by isopycnic density gradient centrifugation until only low levels of contaminating chloroplasts and mitochondria wereExpand
The circadian bioluminescence rhythm of Gonyaulax is related to daily variations in the number of light-emitting organelles.
The number of scintillons, which are cellular organelles responsible for light emission in the marine alga Gonyaulax, were counted by both immunofluorescence and electron microscopic methods andExpand
Circadian regulation of bioluminescence in Gonyaulax involves translational control.
TLDR
The LBP mRNA levels, as determined by in vitro translations and by RNA (Northern) hybridizations, do not vary over the daily cycle, indicating that circadian control of bioluminescence in this species is mediated by translation. Expand
SPECIES OF OCEANIC DINOFLAGELLATES IN THE GENERA DISSODINIUM AND PYROCYSTIS: INTERCLONAL AND INTERSPECIFIC COMPARISONS OF THE COLOR AND PHOTON YIELD OF BIOLUMINESCENCE 1
TLDR
The data suggested that enzyme turnover occurred in the reactions producing light during mechanical stimulation of Dissodinium and Pyrocystis species, and produced much higher numbers of photons per cell nitrogen than Gonyaulax polyedra or Pyrodinium bahamense. Expand
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