THE CHEMISTRY OF INSECT HEMOLYMPH

@article{Wyatt1956THECO,
  title={THE CHEMISTRY OF INSECT HEMOLYMPH},
  author={Gerard R. Wyatt and T. C. Loughheed and S. Silver Wyatt},
  journal={The Journal of General Physiology},
  year={1956},
  volume={39},
  pages={853 - 868}
}
1. Hemolymph was collected for analysis from the silkworm, Bombyx mori, in a series of developmental stages ranging from the second molt to the late pupa. The mean pH of larval hemolymph after collection was found to be 6.45, that of pupal hemolymph, 6.57; in vivo values may be slightly lower. Total dry solids ranged from 5.4 to 10.6 per cent. Total protein ranged from 1.2 to 5.3 per cent, increasing rapidly during the fifth instar. 2. Free amino acids were separated chromatographically and… Expand
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406 Citations

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Protein concentration in the hemolymph of the large milkweed bug, Oncopeltus fasciatus (Dallas).
TLDR
The concentration values reported can be used in comparative studies of protein concentration in insects of known age, sex, developmental stage and nutritional state; for studies on the hemolymph from the milkweed bug under various experimental conditions; and for comparison with other animals. Expand
Changes in the concentration of free amino acids in the haemolymph of Rhodnius prolixus during the fifth instar.
  • F. M. Barrett
  • Biology, Medicine
  • Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1974
TLDR
Changes in other amino acids were less pronounced and did not suggest any obvious connection with specifically identifiable developmental events, but concentrations of proline, leucine, phenylalanine and histidine differ considerably from the only other published quantitative values for R. prolixus haemolymph. Expand
Free amino acids in the haemolymph of Calpodes ethlius during the fifth instar.
TLDR
Glycine undergoes a large and sustained concentration increase approx 48 hr after the onset of wax secretion, a change that is paralleled by increased concentration of a ninhydrin-positive compound tentatively identified as α-amino adipic acid. Expand
Free amino acids of the haemolymph of the cotton leaf-worm, Spodoptera littoralis boisduval larvae (Lepidoptera: Noctuidae)
TLDR
Free amino acids of the haemolymph of the second, third, fourth, fifth and sixth instar larvae of Spodoptera littoralis Boisduval, were determined quantitatively by two-dimensional paper chromatography and histidine, citrulline and γ-aminobutyric acid increased steadily as development proceeded. Expand
Studies on the haemolymph proteins of the blowfly Phormia regina. I. Changes in ontogenetic patterns.
TLDR
Characteristic changes in the ontogenetic patterns have been observed during development, including the appearance of new protein fractions and the disappearance of others in both pupal and adult stages, indicative of the different origin of the respective protein components. Expand
Concentration of insect hemolymph proteins under various experimental conditions.
TLDR
The protein concentration in the hemolymph of fifth instar and adult milkweed bugs was determined under three experimental conditions and the mammalian carcinogen, 9.10-dimethyl-1,2-benzanthracene (DMB), did not have any effect on the Hemolymph protein. Expand
The haemolymph of the sphingidae moth Erinnyis ello
TLDR
The greenish-yellow color of the haemolymph is due to isozeaxanthin bound to a protein which migrates slowly during polyacrylamide gel electrophoresis performed under standard conditions. Expand
Physical properties and chemical composition of the hemolymph of Rhynchosciara americana (Diptera) larvae.
TLDR
A chemical analysis of Rhynchosciara americana larva hemolymph accounting for 88 per cent of the total blood dry weight was carried out and the ionic pattern established after Na, K, Mg, Ca, Fe, Cu, Zn and Cl determinations is different from that of other Diptera. Expand
Haemolymph proteins of the honey bee—I. Separation and characterization of haemolymph proteins of worker larvae
Abstract 1. 1. Characterization of haemolymph proteins of honey bee worker larvae by several electrophoretic methods and by ultracentrifugation revealed that they resemble by these criteria the human… Expand
On the free amino acids in the haemolymph of a millipede
1. 1. Chromatographic studies on the free amino acids in the blood of the millipede Jonespeltis splendidus revealed cystine, lysine, arginine, asparagine, serine, glycine, hydroxyproline, threonine,… Expand
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