TFIIS enhances transcriptional elongation through an artificial arrest site in vivo.

@article{Kulish2001TFIISET,
  title={TFIIS enhances transcriptional elongation through an artificial arrest site in vivo.},
  author={D Kulish and Kevin Struhl},
  journal={Molecular and cellular biology},
  year={2001},
  volume={21 13},
  pages={4162-8}
}
Transcriptional elongation by RNA polymerase II has been well studied in vitro, but understanding of this process in vivo has been limited by the lack of a direct and specific assay. Here, we designed a specific assay for transcriptional elongation in vivo that involves an artificial arrest (ARTAR) site designed from a thermodynamic theory of DNA-dependent transcriptional arrest in vitro. Transcriptional analysis and chromatin immunoprecipitation experiments indicate that the ARTAR site can… CONTINUE READING