TEMPORAL AND SPATIAL GENE EXPRESSION OF Ole e 3 ALLERGEN IN OLIVE ( OLEA EUROPAEA L . ) POLLEN

@inproceedings{Alch2004TEMPORALAS,
  title={TEMPORAL AND SPATIAL GENE EXPRESSION OF Ole e 3 ALLERGEN IN OLIVE ( OLEA EUROPAEA L . ) POLLEN},
  author={Juan de Dios Alch{\'e} and In{\'e}s Adriana Cismondi and Antonio Jes{\'u}s Castro and ABDEL MOUNIM HAMMAN-KHALIFA and MARĺA ISABEL RODRĺGUEZ-GARCĺA},
  year={2004}
}
This paper describes the distribution of Ole e 3 allergen and its transcripts in the developing anther and in mature olive pollen. Northern blot and RT-PCR analyses over the course of pollen development show that Ole e 3 transcripts accumulate exclusively in mature pollen. This gene therefore corresponds to a "late gene." The sequences amplified by RT-PCR display high identity with those already reported for Ole e 3, including two Ca-binding motifs. Immunoblot analysis of the allergen shows… CONTINUE READING
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