TEM beta-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis.

@article{Raquet1994TEMBM,
  title={TEM beta-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis.},
  author={Xavier Raquet and Josette Lamotte-Brasseur and Eveline Fonz{\'e} and Sylvie Goussard and Patrice Courvalin and Jean Marie Fr{\`e}re},
  journal={Journal of molecular biology},
  year={1994},
  volume={244 5},
  pages={
          625-39
        }
}
The catalytic properties of six "natural" mutants of the TEM-1 beta-lactamase have been studied in detail, with special emphasis on their activity versus third-generation cephalosporins. On the basis of the recently determined high-resolution structure of the wild-type enzyme, and of the substrates' structures optimized by the AMI quantum chemistry method, we have attempted to explain the influences of the mutations on the substrate profiles of the enzymes. Some of the kinetic results have thus… CONTINUE READING
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