TECHNICAL BRIEF: Optimized pipeline for isolation of high-quality RNA from corneal cell subpopulations

@inproceedings{Bath2014TECHNICALBO,
  title={TECHNICAL BRIEF: Optimized pipeline for isolation of high-quality RNA from corneal cell subpopulations},
  author={Chris Bath and Trine Fink and Henrik Vorum and Jesper {\O}stergaard Hjortdal and Vladimir Zachar},
  booktitle={Molecular vision},
  year={2014}
}
PURPOSE Attempts to determine the transcriptional profile of discrete subsets of limbal epithelial cells in situ using laser capture microdissection (LCM) face two major challenges. First, the transcriptional profile of cells within a tissue may rapidly change as the tissue is excised and exposed to cold ischemia. Second, there is a risk of degradation of the RNA as the cellular compartment is separated from the remaining tissue. An optimized protocol for LCM of corneal epithelium is presented… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 13 REFERENCES

: role of the niche microenvironment

G Pellegrini, CE Traverso, +3 authors M DeLuca
  • 2012

Lasercapture microdissection

V Espina, JD Wulfkuhle, +6 authors LA. Liotta
  • Nat Protoc
  • 2006
VIEW 1 EXCERPT