TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor shedding during detachment.

@article{Dustin1996TCRmediatedAO,
  title={TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor shedding during detachment.},
  author={Michael L. Dustin and Julie Miller and Sheila Ranganath and Dario A A Vignali and Nicholas J. Viner and Christopher A. Nelson and Emil R Unanue},
  journal={Journal of immunology},
  year={1996},
  volume={157 5},
  pages={2014-21}
}
T cell recognition of foreign Ag/MHC class II complexes is sensitive down to approximately 100 complexes per cell or approximately 0.2 complexes/micron2. To better understand the physical basis of the recognition stage of Ag presentation, we examined adhesion of the lysozyme- specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules. Adhesion of 3A9 cells required a superphysiologic density of the MHC class II/peptide complex… CONTINUE READING