TCR+/BCR+ dual-expressing cells and their associated public BCR clonotype are not enriched in type 1 diabetes

  title={TCR+/BCR+ dual-expressing cells and their associated public BCR clonotype are not enriched in type 1 diabetes},
  author={Alberto Sada Japp and Wenzhao Meng and Aaron M. Rosenfeld and D. Perry and Puchong Thirawatananond and Rhonda Bacher and Chengyang Liu and Jay Gardner and Mark A. Atkinson and Klaus H. Kaestner and Todd M. Brusko and Ali Naji and Eline T. Luning Prak and Michael R. Betts},
4 Citations
CD5‐expressing CD8+ T‐cell subsets differ between children with type 1 diabetes and controls
Comparisons of conventional immune cells detected increased proportions of CD3+ T cells in T1D patients, whereas CD19+ B‐cell proportions were comparable to controls, and in vitro experiments showed stable CD5 expression differences of CD8- T cells after T‐cell activation, cytokine stimulation and culture.
Insights From Single Cell RNA Sequencing Into the Immunology of Type 1 Diabetes- Cell Phenotypes and Antigen Specificity
A review of the first papers to use scRNAseq to characterise human lymphocyte phenotypes in T1D in the peripheral blood, pancreatic lymph nodes and islets to identify novel biomarkers and targets for immunotherapy.
Single cell based high-throughput Ig and TCR repertoire sequencing analysis in rhesus macaques
The ability to perform high-throughput immune repertoire analysis in rhesus macaques at the single cell level is established.


Tissue distribution and clonal diversity of the T and B cell repertoire in type 1 diabetes.
A highly tissue-restricted CD4+ repertoire, while up to 24% of CD8+ clones were shared among tissues, supporting the utility of coupling immunosequencing data with knowledge of characterized autoreactive receptors.
Synthetic CD4+ T cell-targeted antigen-presenting cells elicit protective antitumor responses.
The data support the use of cell-free, synthetic aAPC as a specific and versatile alternative to expand peptide-specific CD4(+) T cells in adoptive and active immunotherapy.
Computational Evaluation of B-Cell Clone Sizes in Bulk Populations
A series of pragmatic analytical approaches are provided for estimating B cell clone size in NGS immune repertoire profiling data of antibody heavy chain gene rearrangements by next-generation sequencing.
Reprogramming human B cells into induced pluripotent stem cells and its enhancement by C/EBPα
It is shown for the first time that freshly isolated non-cultured human cord blood (CB)- and peripheral blood (PB)-derived CD19+CD20+ B cells can be reprogrammed to iPSCs carrying complete VDJH immunoglobulin (Ig) gene monoclonal rearrangements using non-integrative tetracistronic, but not monocistronic, OSKM-expressing Sendai Virus.
Convergent recombination shapes the clonotypic landscape of the naïve T-cell repertoire
The key predictions of convergent recombination are tested in a comprehensive evaluation of the naïve CD8+ TCRβ repertoire in mice and provide a framework for understanding the early mobilization of public CD8-cell clonotypes, which can exert profound biological effects during acute infectious processes.
The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice
A small subpopulation of normal murine splenic B cells carrying all of the classic B cells markers (IgM, IgD, Ia, and ThB) also carries Ly-1, one of the major T cell surface molecules, which accounts for the previously reported "spontaneous" IgM secretion by NZB spleen cells in culture.
Conversion of mature B cells into T cells by dedifferentiation to uncommitted progenitors
It is shown that conditional Pax5 deletion in mice allowed mature B cells from peripheral lymphoid organs to dedifferentiate in vivo back to early uncommitted progenitors in the bone marrow, which rescued T lymphopoiesis in the thymus of T-cell-deficient mice and uncovered an extraordinary plasticity of mature peripheral B cells despite their advanced differentiation stage.
Commitment to the B-lymphoid lineage depends on the transcription factor Pax5
It is shown that pro-B cells lacking Pax5 are also incapable of in vitro B-cell differentiation unless Pax5 expression is restored by retroviral transduction, and Pax5 plays an essential role in B-lineage commitment by suppressing alternative lineage choices.
Successful generation of primary virus-specific and anti-tumor T-cell responses from the naïve donor T-cell repertoire is determined by the balance between antigen-specific precursor T cells and regulatory T cells
It is demonstrated that the likelihood of successful generation of primary immune responses is determined by a delicate balance between the numbers of antigen-specific precursor T cells and the numbers and activation state of regulatory T cells locally at the site of priming of the immune response.
The molecular basis for public T-cell responses?
It is proposed that the frequency of production of T cells bearing different T CRs during recombination has an important role in the sharing of TCRs in an immune response, with variable levels of 'convergent recombination' driving production frequencies.