TAT protein transduction into isolated perfused hearts: TAT-apoptosis repressor with caspase recruitment domain is cardioprotective.

@article{Gustafsson2002TATPT,
  title={TAT protein transduction into isolated perfused hearts: TAT-apoptosis repressor with caspase recruitment domain is cardioprotective.},
  author={{\AA} B Gustafsson and M. Richard Sayen and Scott D Williams and Michael T. Crow and Roberta A Gottlieb},
  journal={Circulation},
  year={2002},
  volume={106 6},
  pages={735-9}
}
BACKGROUND Linkage of the 11-amino-acid transduction domain of HIV TAT to a heterologous protein allows the protein to be transduced readily into cells. METHODS AND RESULTS In this study, we inserted the apoptosis repressor with caspase recruitment domain (ARC) or beta-galactosidase (beta-gal) cDNA into the pTAT-hemagglutinin bacterial expression vector to produce genetic in-frame TAT-ARC or TAT-beta-gal fusion proteins for use in cell culture and in Langendorff perfusion of adult rat hearts… CONTINUE READING

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