TAT-phiC31 integrase mediates DNA recombination in mammalian cells.

@article{Zhang2009TATphiC31IM,
  title={TAT-phiC31 integrase mediates DNA recombination in mammalian cells.},
  author={Mao-xiang Zhang and Zhi-hui Li and Yu-xiang Fang and Huan-zhang Zhu and Jing-lun Xue and Jin-zhong Chen and William WeiGuo Jia},
  journal={Journal of biotechnology},
  year={2009},
  volume={142 2},
  pages={
          107-13
        }
}
Streptomyces phage integrase phiC31 is capable of mediating site-specific insertions in mammalian genomes. To avoid potential toxicity of long-term expression of phiC31 in host cells, we developed a method employing a cell-permeable TAT-phiC31 integrase. His6-tagged phiC31 proteins with or without an HIV TAT intercellular transducing peptide were generated and purified. Both of them retained integrase activity in vitro. However, TAT-phiC31 but not phiC31 was able to mediate a specific… CONTINUE READING
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