TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.

@article{Young2003TAMSTF,
  title={TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.},
  author={Lei Young and Qihan Dong},
  journal={Nucleic acids research},
  year={2003},
  volume={31 3},
  pages={e11}
}
Site-directed mutagenesis is an invaluable tool for functional studies and genetic engineering. However, most current protocols require the target DNA to be cloned into a plasmid vector before mutagenesis can be performed, and none of them are effective for multiple-site mutagenesis. We now describe a method that allows mutagenesis on any DNA template (eg. cDNA, genomic DNA and plasmid DNA), and is highly efficient for multiple-site mutagenesis (up to 100%). The technology takes advantage of… CONTINUE READING