TALEs of genome targeting

@article{Boch2011TALEsOG,
  title={TALEs of genome targeting},
  author={Jens Boch},
  journal={Nature Biotechnology},
  year={2011},
  volume={29},
  pages={135-136}
}
  • J. Boch
  • Published 1 February 2011
  • Biology
  • Nature Biotechnology
New tools for site-specific genome targeting in human cells are generated from TALE proteins. 

Genome Editing for the Generation of Immunodeficient Pigs

During this study, genome editing tools were applied for the modification of three genes playing crucial roles in the immune system, with the aim of generating immunodeficient pigs.

Discovery of Synthetic Lethal Interactions with CDH1 in Medulloblastoma

The water needs of this region have changed in recent years from being primarily for agricultural purposes to domestic and industrial uses now, and the number of wells has declined in both the agricultural and industrial areas.

The transience of transient overexpression

Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells, but genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.

Designing Non-viral Targeted Integrating Vectors for Genome Engineering in Vertebrates

Several efforts during the last decade led to the development of nonviral approaches based on DNA-modifying enzymes by exploiting the cellular mechanisms of cut-and-paste transposition and homologous recombination.

Safe harbours for the integration of new DNA in the human genome

'genomic safe harbours' are discussed — chromosomal locations where therapeutic transgenes can integrate and function in a predictable manner without perturbing endogenous gene activity and promoting cancer.

Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation

It is shown that catalytically dead Cas9 (dCas9) targeted to genomic regions upstream or downstream of the transcription start site allows for specific and sustainable gene-expression level alterations in tumor cells in vitro and in syngeneic immune-competent mouse models.

Gene delivery methods and genome editing of human pluripotent stem cells.

Precision genetic modifications: a new era in molecular biology and crop improvement

Improvement of the efficiency and precision of PGM techniques will enable researchers to precisely alter gene expression and biological/chemical pathways, probe gene function, modify epigenetic marks and improve crops by increasing yield, quality and tolerance to limiting biotic and abiotic stress conditions.
...

References

SHOWING 1-10 OF 21 REFERENCES

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

A new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease is reported.

A TALE nuclease architecture for efficient genome editing

This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.

Genome editing with engineered zinc finger nucleases

A broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair.

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.

TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain

The creation and initial characterization of a group of rare-cutting, site-specific DNA nucleases produced by fusion of the restriction enzyme FokI endonuclease domain (FN) with the high-specificity DNA-binding domains of AvrXa7 and PthXo1 are reported.

A Simple Cipher Governs DNA Recognition by TAL Effectors

It is shown that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence, which represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effects in research and biotechnology.

A Bacterial Effector Acts as a Plant Transcription Factor and Induces a Cell Size Regulator

It is shown that AvrBs3 induces the expression of a master regulator of cell size, upa20, which encodes a transcription factor containing a basic helix-loop-helix domain that provokes developmental reprogramming of host cells by mimicking eukaryotic transcription factors.

Xanthomonas AvrBs3 family-type III effectors: discovery and function.

The discovery of TAL effectors is described, which act as transcriptional activators in the plant cell nucleus and are determined by a novel modular DNA-binding domain.

Genetic and structural characterization of the avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria

SummaryThe avirulence gene avrBs3 from Xanthomonas campestris pv. vesicatoria was cloned and found to be localized on a self-transmissable plasmid. Genetic analysis of an avrBs3 insertion mutation

Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors

The data demonstrate that the TALE scaffold can be tailored to target user-defined DNA sequences in whole genomes and mediates specific interaction with G nucleotides that thus far could not be targeted specifically by any known RVD type.