TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish.


Transcription activator-like effector nucleases (TALENs) are engineered endonucleases composed of a customized transcription activator-like effector (TALE) DNA-binding domain and a FokI DNA cleavage domain. TALENs induce DNA double-strand breaks (DSBs) at their target sites on the chromosome and have been successfully used for genome engineering in many… (More)
DOI: 10.1016/j.ymeth.2014.02.010


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