TALEN-based knockout library for human microRNAs

  title={TALEN-based knockout library for human microRNAs},
  author={Young-Kook Kim and Gabbine Wee and Joha Park and Jongkyu Kim and Daehyun Baek and Jin-Soo Kim and V. Narry Kim},
  journal={Nature Structural &Molecular Biology},
Various technical tools have been developed to probe the functions of microRNAs (miRNAs), yet their application has been limited by low efficacy and specificity. To overcome the limitations, we used transcription activator–like effector nucleases (TALENs) to knock out human miRNA genes. We designed and produced a library of 540 pairs of TALENs for 274 miRNA loci, focusing on potentially important miRNAs. The knockout procedure takes only 2–4 weeks and can be applied to any cell type. As a case… CONTINUE READING

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