TALE nickase mediates high efficient targeted transgene integration at the human multi-copy ribosomal DNA locus.

@article{Wu2014TALENM,
  title={TALE nickase mediates high efficient targeted transgene integration at the human multi-copy ribosomal DNA locus.},
  author={Yong Wu and Tieli Gao and Xiaolin Wang and Youjin Hu and Xuyun Hu and Zhiqing Hu and Jialun Pang and Zhuo Li and Jinfeng Xue and Mai Feng and Lingqian Wu and Desheng Liang},
  journal={Biochemical and biophysical research communications},
  year={2014},
  volume={446 1},
  pages={
          261-6
        }
}
Although targeted gene addition could be stimulated strikingly by a DNA double strand break (DSB) created by either zinc finger nucleases (ZFNs) or TALE nucleases (TALENs), the DSBs are really mutagenic and toxic to human cells. As a compromised solution, DNA single-strand break (SSB) or nick has been reported to mediate high efficient gene addition but with marked reduction of random mutagenesis. We previously demonstrated effective targeted gene addition at the human multicopy ribosomal DNA… CONTINUE READING

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