TAL effectors mediate high-efficiency transposition of the piggyBac transposon in silkworm Bombyx mori L

@inproceedings{Ye2015TALEM,
  title={TAL effectors mediate high-efficiency transposition of the piggyBac transposon
in silkworm Bombyx mori L},
  author={Lupeng Ye and Zhengying You and Qiujie Qian and Yuyu Zhang and Jiaqian Che and J. -G. Song and Boxiong Zhong},
  booktitle={Scientific reports},
  year={2015}
}
The piggyBac (PB) transposon is one of the most useful transposable elements, and has been successfully used for genetic manipulation in more than a dozen species. However, the efficiency of PB-mediated transposition is still insufficient for many purposes. Here, we present a strategy to enhance transposition efficiency using a fusion of transcription activator-like effector (TALE) and the PB transposase (PBase). The results demonstrate that the TALE-PBase fusion protein which is engineered in… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 20 references

Postintegration stability of the silkworm piggyBac transposon.

Insect biochemistry and molecular biology • 2014

Genetic marking of sex using a W chromosome-linked transgene.

Insect biochemistry and molecular biology • 2013

Identification of a midgut-specific promoter in the silkworm Bombyx mori

L Jiang
Biochem Bioph Res Co 433, • 2013

Similar Papers

Loading similar papers…